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Registros recuperados: 16
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Caloranaerobacter azorensis gen. nov., sp nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent ArchiMer
Wery, Nathalie; Moricet, Jean-marc; Cueff, Valerie; Jean, Joelle; Pignet, Patricia; Lesongeur, Francoise; Cambon-bonavita, Marie-anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic bacterium, designated MV1087(T), was isolated from a deep-sea hydrothermal chimney sample collected from the Mid-Atlantic Ridge. The cells were straight, motile and stained Gram-negative. Growth was observed from 45 to 65 degreesC, with an optimum around 65 degreesC. No growth was observed at 40 or 70 degreesC. Growth was observed from pH 5.5 to 9.0 and the optimum pH was around 7. The salinity range for growth was 10-100 g sea salt l(-1) (corresponding to 6.5-65 g NaCl l(-1)) with an optimum at 30 g sea salt l(-1) (20 g NaCl l(-1)). Strain MV1087(T) was heterotrophic, able to ferment proteinaceous substrates, such as brain/heart infusion and gluten, and carbohydrates, such as glucose, xylan and starch. The...
Tipo: Text Palavras-chave: Thermophile; Deep sea vent; Caloranaerobacter azorensis; Caloranaerobacter; Bacteria.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-586.pdf
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Characterization of the antifungal activity of Lactobacillus harbinensis K.V9.3.1Np and Lactobacillus rhamnosus K.C8.3.1I in yogurt ArchiMer
Delavenne, Emilie; Cliquet, Sophie; Trunet, Clément; Barbier, Georges; Mounier, Jérome; Le Blay, Gwenaelle.
Few antifungal protective cultures adapted to fermented dairy products are commercially available because of the numerous constraints linked to their market implementation. Consumer’s demand for naturally preserved food products is growing and the utilization of lactic acid bacteria is a promising way to achieve this goal. In this study, using a 25-1 factorial fractional design, we first evaluated the effects of fermentation time, of initial sucrose concentration and of the initial contamination amount of a spoilage yeast, on antifungal activities of single and mixed cultures of Lactobacillus rhamnosus K.C8.3.1I and Lactobacillus harbinensis K.V9.3.1Np in yogurt. L. harbinensis K.V9.3.1Np, the most relevant strain with regard to antifungal activity was...
Tipo: Text Palavras-chave: Lactobacillus harbinensis; Bioprotective culture; Antifungal activity.
Ano: 2015 URL: http://archimer.ifremer.fr/doc/00189/30025/28510.pdf
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Clostridium caminithermale sp nov., a slightly halophilic and moderately thermophilic bacterium isolated from an Atlantic deep-sea hydrothermal chimney ArchiMer
Brisbarre, Nadège; Fardeau, Marie-laure; Cueff, Valerie; Cayol, Jean-luc; Barbier, Georges; Cilia, Valérie; Ravot, Gilles; Thomas, Pierre; Garcia, Jean-louis; Ollivier, Bernard.
A strictly anaerobic, slightly halophilic and moderately thermophilic, sporulating rod designated strain DVird3(T) was isolated from deep-sea hydrothermal vent samples collected at a depth of approximately 800 m on the Atlantic Ocean Ridge. Strain DVird3(T) possessed a few laterally inserted flagella, had a DNA G+C content of 33.1 mol% and grew optimally at pH 6.6 and at 45 degreesC. Growth was observed at temperatures between 20 and 58 degreesC and at pH values between 5.8 and 8.2. The optimum NaCl concentration for growth was 3% sea salt (30 g l(-1)); no growth was observed in the presence of 15 or 60 g sea salt l(-1). Strain DVird3(T) is heterotrophic and utilizes some sugars and various single amino acids. Acetate was the main fatty acid detected from...
Tipo: Text Palavras-chave: Phylogeny; Thermophile; Bacteria; Deep sea hydrothermal vent.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/publication-588.pdf
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Copper levels evolution in Arcachon bay oysters : role of the regulation on antifouling paints ArchiMer
Alzieu, Claude; Barbier, Georges; Sanjuan, Jane.
The ban of tributyltin compounds in antifouling paints induced a strong decrease in the level of tin in Arcachon bay. At the same time, copper inputs have increased tenfold since 1982 owing to the widespread use of paints containing cuprous oxide. In spite of this large input, the statistical analysis of monitoring data (1979-1985) show a relative stability in the copper levels in Arcachon bay oysters.
Tipo: Text Palavras-chave: Cuivre; Huîtres; Bassin d'Arcachon; Peintures antisalissures; Copper; Oysters; Arcachon Bay; Antifouling paints.
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00022/13291/10337.pdf
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Des enzymes pour l'industrie ArchiMer
Querellou, Joel; Gueguen, Yannick; Barbier, Georges; Dietrich, Jacques.
Tipo: Text
Ano: 1999 URL: http://archimer.ifremer.fr/doc/00092/20334/18037.pdf
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Evolution des teneurs en cuivre des huîtres du Bassin d'Arcachon: influence de la législation sur les peintures antisalissures ArchiMer
Alzieu, Claude; Barbier, Georges; Sanjuan, Jane.
The ban of tributyltin compounds in antifouling paints induced a strong decrease in the level of tin in Arcachon bay. At the same time, copper inputs have increased tenfold since 1982 owing to the widespread use of paints containing cuprous oxide. In spite of this large input, the statistical analysis of monitoring data (1979-1985) show a relative stability in the copper levels in Arcachon bay oysters.
Tipo: Text
Ano: 1987 URL: http://archimer.ifremer.fr/doc/00155/26605/25506.pdf
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In-depth analyses of deep subsurface sediments using 454-pyrosequencing reveals a reservoir of buried fungal communities at record-breaking depths ArchiMer
Redou, Vanessa; Ciobanu, Maria Cristina; Pachiadaki, Maria G.; Edgcomb, Virginia; Alain, Karine; Barbier, Georges; Burgaud, Gaetan.
The deep subseafloor, extending from a few centimeters below the sediment surface to several hundred meters into sedimentary deposits, constitutes the deep biosphere and harbors an unexpected microbial diversity. Several studies have described the occurrence, turnover, activity and function of subseafloor prokaryotes; however, subsurface eukaryotic communities still remain largely underexplored. Ribosomal RNA surveys of superficial and near-surface marine sediments have revealed an unexpected diversity of active eukaryotic communities, but knowledge of the diversity of deep subseafloor microeukaryotes is still scarce. Here, we investigated the vertical distribution of DNA and RNA fungal signatures within subseafloor sediments of the Canterbury basin (New...
Tipo: Text Palavras-chave: 454 pyrotag; Canterbury basin; Deep subseafloor; Fungi; Microeukaryotes.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00247/35810/34418.pdf
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Le littoral de la région Nord-Pas de Calais - qualité du milieu marin ArchiMer
Barbier, Georges; Arnal, Olivier; Delattre, Jean-marie; Grossel, Hubert; Morel, Marc.
Tipo: Text
Ano: 1986 URL: http://archimer.ifremer.fr/doc/00083/19377/16971.pdf
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Marine culturable yeasts in deep-sea hydrothermal vents: species richness and association with fauna ArchiMer
Burgaud, Gaetan; Arzur, Danielle; Durand, Lucile; Cambon-bonavita, Marie-anne; Barbier, Georges.
Investigations of the diversity of culturable yeasts at deep-sea hydrothermal sites have suggested possible interactions with endemic fauna. Samples were collected during various oceanographic cruises at the Mid-Atlantic Ridge, South Pacific Basins and East Pacific Rise. Cultures of 32 isolates, mostly associated with animals, were collected. Phylogenetic analyses of 26S rRNA gene sequences revealed that the yeasts belonged to Ascomycota and Basidiomycota phyla, with the identification of several genera: Rhodotorula, Rhodosporidium, Candida, Debaryomyces and Cryptococcus. Those genera are usually isolated from deep-sea environments. To our knowledge, this is the first report of yeasts associated with deep-sea hydrothermal animals.
Tipo: Text Palavras-chave: Yeasts; Hydrothermal vents; Fauna; 26S rRNA gene; FISH.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11704/8475.pdf
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Marinitoga camini gen, nov., sp nov., a rod-shaped bacterium belonging to the order Thermotogales, isolated from a deep-sea hydrothermal vent ArchiMer
Wery, Nathalie; Lesongeur, Francoise; Pignet, Patricia; Derennes, Veronique; Cambon-bonavita, Marie-anne; Godfroy, Anne; Barbier, Georges.
A thermophilic, anaerobic, chemo-organotrophic sulfur-reducing bacterium, designated MV1075(T), was isolated from a deep-sea hydrothermal chimney sample collected on the Mid-Atlantic Ridge. Cells were rod-shaped with a sheath-like outer structure, motile with polar flagella and stained Gramnegative. They appeared singly, in pairs or in short chains. The temperature range for growth was 25-65 degreesC, with an optimum at 55 degreesC. Growth was observed from ph 5 to ph 9, and the optimum ph was around 7. The salinity range for growth was 15-70 g sea salt I-1 (corresponding to 10-45 g NaCl I-1), with an optimum at 30 g I-1 (20 g NaCl I-1). The isolate was able to grow on a broad spectrum of carbohydrates or complex proteinaceous substrates. Sulfur was not...
Tipo: Text Palavras-chave: Marinitoga camini; Thermotogales; Thermophile; Deep sea hydrothermal vent.
Ano: 2001 URL: http://archimer.ifremer.fr/doc/2001/publication-587.pdf
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Microorganisms persist at record depths in the subseafloor of the Canterbury Basin ArchiMer
Ciobanu, Maria Cristina; Burgaud, Gaetan; Dufresne, Alexis; Breuker, Anja; Redou, Vanessa; Ben Maamar, Sarah; Gaboyer, Frederic; Vandenabeele-trambouze, Odile; Lipp, Julius Sebastian; Schippers, Axel; Vandenkoornhuyse, Philippe; Barbier, Georges; Jebbar, Mohamed; Godfroy, Anne; Alain, Karine.
The subsurface realm is colonized by microbial communities to depths of >1000 meters below the seafloor (m.b.sf.), but little is known about overall diversity and microbial distribution patterns at the most profound depths. Here we show that not only Bacteria and Archaea but also Eukarya occur at record depths in the subseafloor of the Canterbury Basin. Shifts in microbial community composition along a core of nearly 2 km reflect vertical taxa zonation influenced by sediment depth. Representatives of some microbial taxa were also cultivated using methods mimicking in situ conditions. These results suggest that diverse microorganisms persist down to 1922 m.b.sf. in the seafloor of the Canterbury Basin and extend the previously known depth limits of...
Tipo: Text Palavras-chave: Deep biosphere; Subsurface life; Eukaryote; Record depth.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00201/31243/29895.pdf
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New information on the ecology of deep-sea vent communities in the azores triple junction area : preliminery results of the Diva 2 cruise (May 31-July 4, 1994) ArchiMer
Desbruyeres, Daniel; Alayse, Anne-marie; Antoine, Elisabeth; Barbier, Georges; Barriga, Fjas; Biscoito, Manuel; Briand, Patrick; Brulport, Jean-pierre; Comtet, Thierry; Cornec, Laurence; Crassous, Philippe; Dando, Paul; Fabri, Marie-claire; Felbeck, Horst; Lallier, Francois; Fiala Medioni, Aline; Goncalves, Joao; Menard, Frederic; Kerdoncuff, Jacques; Patching, John; Saldanha, Luis; Sarradin, Pierre-marie.
Tipo: Text
Ano: 1994 URL: http://archimer.ifremer.fr/doc/00179/29005/27432.pdf
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Phylogenetic characterization of the bacterial assemblage associated with mucous. secretions of the hydrothermal vent polychaete Paralvinella palmiformis ArchiMer
Alain, Karine; Olagnon, Michel; Desbruyeres, Daniel; Page, Antoine; Barbier, Georges; Juniper, Kim; Querellou, Joel; Cambon-bonavita, Marie-anne.
As part of an ongoing examination of microbial diversity associated with hydrothermal vent polychaetes of the family Alvinellidae, we undertook a culture-independent molecular analysis of the bacterial assemblage associated with mucous secretions of the Northeastern Pacific vent polychaete Paralvinella palmiformis. Using a molecular 16S rDNA-based phylogenetic approach, clone libraries were constructed from two samples collected from active sulfide edifices in two hydrothermal vent fields. In both cases, clone libraries were largely dominated by epsilon-Proteobacteria. Phylotypes belonging to the Cytophaga-Flavobacteria and to the Verrucomicrobia were also largely represented within the libraries. The remaining sequences were related to the taxonomic...
Tipo: Text Palavras-chave: Proteobacteria; 16S rRNA; Microbial diversity; Phylogeny; Hydrothermal vent; Paralvinella palmiformis.
Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-661.pdf
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Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections ArchiMer
Alexandre, Youenn; Le Berre, Rozenn; Barbier, Georges; Le Blay, Gwenaelle.
Background: Pseudomonas aeruginosa is an opportunistic pathogen that significantly increases morbidity and mortality in nosocomial infections and cystic fibrosis patients. Its pathogenicity especially relies on the production of virulence factors or resistances to many antibiotics. Since multiplication of antibiotic resistance can lead to therapeutic impasses, it becomes necessary to develop new tools for fighting P. aeruginosa infections. The use of probiotics is one of the ways currently being explored. Probiotics are microorganisms that exert a positive effect on the host's health and some of them are known to possess antibacterial activities. Since most of their effects have been shown in the digestive tract, experimental data compatible with the...
Tipo: Text Palavras-chave: Pseudomonas aeruginosa; Lactobacillus; Probiotics; Organic acids; Biofilm formation; Elastolytic activity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00197/30842/29211.pdf
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Tepidibacter formicigenes sp nov., a novel spore-forming bacterium isolated from a Mid-Atlantic Ridge hydrothermal vent ArchiMer
Urios, Laurent; Cueff, Valerie; Pignet, Patricia; Barbier, Georges.
A novel anaerobic, Gram-positive, sporulating and strictly chemoorganoheterotrophic bacterium, designated strain DV1 184 T, was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were short, straight rods (4 mum long and 0.8 mum wide) and were motile with peritrichous flagella. They grew between 35 and 55 degreesC (optimum, 45 degreesC), between pH 5.0 and 8.0 (optimum, 6.0) and at 20-60 g sea salts l(-1) (optimum sea salts concentration, 30 g l(-1)). Strain DV1184(T) was able to ferment yeast extract, tryptone, peptone, glucose, sucrose, maltose and pyruvate. The main fermentation products from glucose were (in decreasing order) formate, acetate and ethanol. The genomic DNA G+C content was 29 mol%. Phylogenetic...
Tipo: Text Palavras-chave: Phylogeny; Bacteria; Deep sea hydrothermal vent.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-589.pdf
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Thermosipho atlanticus sp nov., a novel member of the Thermotogales isolated from a Mid-Atlantic Ridge hydrothermal vent ArchiMer
Urios, Laurent; Cueff-gauchard, Valerie; Pignet, Patricia; Postec, Anne; Fardeau, Marie-laure; Ollivier, Bernard; Barbier, Georges.
A novel anaerobic, thermophilic and heterotrophic bacterium, designated strain DV114O(T), was isolated from a deep-sea hydrothermal vent sample from the Mid-Atlantic Ridge. The cells were non-motile straight rods, 1-8 mum long and 0-4 mum wide, surrounded by an outer sheath-like structure (toga). They grew at 45-80 degreesC (optimum 65 degreesC), pH 5-0-9-0 (optimum pH 6-0) and at sea salt concentrations of 20-60 gl(-1) (optimum 30 gl(-1)). Strain DV1140(T) was able to ferment yeast extract, peptone, brain heart infusion, gelatin, starch, galactose, arabinose, glucose, trehalose and cellobiose. The fermentation products identified on glucose in the presence of yeast extract and peptone were acetate, isovalerate and hydrogen. The G + C content of the...
Tipo: Text Palavras-chave: Marine microorganisms; Thermosipho atlanticus; Thermotogales; Thermophile; Deep sea vent.
Ano: 2004 URL: http://archimer.ifremer.fr/doc/2004/publication-362.pdf
Registros recuperados: 16
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